Aseptic Technique Diagram
Depicted below are the ingredients used to prepare each of the three environments for culturing bacteria. (Nutrient Agar and Mannitol Salt Agar are both used to prepare agar plates/slants however certain bacteria may not grow in the Mannitol Salt Agar due to their nutritional needs.) From the original stock we transferred the bacteria creating a streak plate on the Agar Plate.
Day 1
To reinforce aseptic technique (see image at left), learn how to properly swab and observe bacterial growth we obtained two environmental samples: our own fingerprints and Cara Egan’s arm cast. To gather bacteria from Cara’s cast, swab was first immersed in a test tube of PBS, swabbed the cast to obtain bacteria and finally created a streak plate (see below for description). To obtain fingerprint bacteria we pressed our fingers on an Agar Plate then washed our hands and repeated to see the difference in bacteria growth. Both were stored upside down and incubated overnight.
To reinforce aseptic technique (see image at left), learn how to properly swab and observe bacterial growth we obtained two environmental samples: our own fingerprints and Cara Egan’s arm cast. To gather bacteria from Cara’s cast, swab was first immersed in a test tube of PBS, swabbed the cast to obtain bacteria and finally created a streak plate (see below for description). To obtain fingerprint bacteria we pressed our fingers on an Agar Plate then washed our hands and repeated to see the difference in bacteria growth. Both were stored upside down and incubated overnight.
Each lab group was given an unknown sample of bacteria to perform various tests on to determine its identity. We were given sample #5. From the original stock of sample we, by means of aseptic transfer, transferred the bacteria to an Agar Plate, Agar Slant test tube and nutrient broth to culture new bacteria.
Depicted below are the ingredients used to prepare each of the three environments for culturing bacteria. (Nutrient Agar and Mannitol Salt Agar are both used to prepare agar plates/slants however certain bacteria may not grow in the Mannitol Salt Agar due to their nutritional needs.) From the original stock we transferred the bacteria creating a streak plate on the Agar Plate.
Using aseptic technique we transferred bacteria by smearing bacteria in the first quadrant then sterilizing the loop and continuing on into the second quadrant. Repeat process of sterilizing and smearing in all four quadrants. Then cover Agar Plate and store upside down in the 37degrees C incubator overnight. To prepare the Slant Agar test tube, again using aseptic technique smear bacteria in test tube and incubate at 37degrees C overnight. To prepare the Nutrient Broth, use aseptic technique to transfer bacteria from original stock into broth, swirl loop in broth, then incubate at 37degrees C overnight. Each of these three cultured bacteria will be used in various experiments throughout the minimester.